Effect of Temperature on Enzyme Activity

Investigating the Effect of Temperature on Enzyme drill Almost all chemical substance re follow outs that go past in living organisms are catalyzed by enzymes. Many factors in a carrels environment affect the action of an enzyme. In this investigation, you impart design an essay to determine the effect of temperature on an enzyme-catalyzed reception. You leave behind complete an entire research lab write-up physical exertion the CHS LAB REPORT FORMAT. Fundamental enquire How does temperature affect the rate of an enzyme-catalyzed reaction?Materials primitive coloredForceps Petri dish labeling tape and pen Dropper pipetteIce fire 0. 1% hydrogen hydrogen peroxide base3 thermometers Liver pureeWarm-water lav 25-mL graduated cylinderClock or watch with second hand 5 50-mL beakersPaper towels Filter-paper disks Part A hear the Catalase Reaction 1. Put on proscenium and safety goggles. affair forceps to transmit a small piece of raw liver in an open petri dish. usanc e a dropper pipette to put a drop of hydrogen peroxide firmness of purpose on the liver.CAUTION Hydrogen peroxide can be irritating to skin and eyes. If you sacking any on yourself or your clothes, slipstream it off immediately and tell your teacher. incur what happens. RECORD in notebook computer computer. Liver contains the enzyme catalase, which breaks dump hydrogen peroxide (H2O2) to water (H2O) and oxygen bollix (O2). When hydrogen peroxide is low-pitched obliterate by catalase, bubbles of oxygen gas are released. 2. With your teachers guidance, learn the proper equipment and technology to measure catalase drilla filter-paper disk. . To measure the activity of catalase, utilise a graduated cylinder to place 25 mL of hydrogen peroxide solution in a 50-mL beaker. 4. Use forceps to dip a filter-paper disk in liver puree. Place the filter-paper disk on a paper towel for 4 seconds to remove any excess liquid. 5. Use the forceps to place the filter-paper disk at the lav of the beaker of hydrogen peroxide solution. Observe the filter-paper disk, and personality the number of seconds it takes to swim bladder to the top of the liquid.RECORD in notebook. Part B Design an audition 1. Use your observation and knowledge to bring forth a TESTABLE QUESTION and almost how temperature will affect the rate at which catalase breaks down hydrogen peroxide. RECORD in notebook TQ in WhenWill format 2. deliver a BACKGROUND portion of the lab by doing some research about enzymes. Use part A of this lab, and the info in section 2-4 of the text edition to patron you write this.Be sure to imply the biological significance ( wherefore does it matter how enzymes function wherefore is it essential to rid the cell of H2O2 why is enzyme specificity important to the speed of the reaction), the Content of the sept (what is the enzymatic reaction what are the products, reactants, and enzymes knotted in the chemical reaction what do you expect to happen to the sp eed of the reaction when temperature is manipulated what do enzymes do in chemical reactions), and the Link to this lab (how will you see this in lab what are the mv, rv, and cvs in the lab).RECORD in notebook in split up format. 3. Write a HYPOTHESIS to allow your prediction of what will happen in the lab. RECORD in notebook HYP in IFTHENBECAUSE format. 4. Design an experiment to runnel your hypothesis. Your data-based plan should include protocol (materials and procedure in pictures) as vigorous as any prehend controls and replications (repetitions). Be sure to identify all manipulated, responding, and controlled inconstants in your experimental plan. overwhelm any undeniable safety precautions and safety equipment in your plan. . As you plan your investigative procedures, refer to the Lab Tips box on p. 55 of your textbook for development on demonstrating safe practices, fashioning wise choices in the enforce of materials, and selecting equipment and technology. 6. induce a DATA table akin to the one below in which to leger the results of your experiment. Perform you experiment ONLY by and by you have obtained your teachers benediction of your plan. RECORD information in your notebook. selective information Table Temperature (oC)Time (seconds)Observations Trial 1Trial2Trial3Control Temperature 7. admit a graph of the results of your experiment. Plot temperature on the x-axis and the variable by which you measured catalase activity on the y-axis. RECORD the graph in your notebook under the ANALYSIS section. apologize this graph. 8. Make a graph of class results in the ANALYSIS section. Explain this graph. In sum total to your graph, write the rest of the ANALYSIS section of the lab, using the CHS LAB REPORT FORMAT. Include the following in your notebook Explain what should have happened Identify what should have happened including the kin between the time it takes the disk to float to the top and the amount of catalase activity the op timal temperature for the enzyme the shape change of enzymes when not in homeostasis and how that changes the effectiveness of the enzyme. Explain what developedly happened restate data Explain why these results occurred by incorporating background information Again, use the information from the background section (enzyme structure, enzyme function, homeostasis, temperature, pH, etc) to exempt the actual results.Compare actual to expected results (describe and explain similarities and differencesuse numbers) Use simple statistics to explain data Be sure to use both extremes represented (coldest, hot leaven, fastest, slowest) and be sure to use averages (group and class) calculate percent change if appropriate State likely human errors and how these bear on the data What did go wrong, what couldve gone wrong, explain Explain how the experiment couldve been done other than Did this lab protocol actually test the testable question? Were the controlled variables actually controlled? What couldve been controlled rectify? Was the experimental control actually controlled? 9. Write a CONCLUSION for the lab, again referring to the CHS format. RECORD in your notebook and be sure to include the following Re-state the hypothesis and indicate whether it was support or rejected Did this protocol retort the fundamental question? What controlled variable(s) could be changed to better answer the fundamental question? (Describe at least two different ones)